Statistical model was proposed with an objective of optimizing enzymatic inulin hydrolytic process. Immobilized biocatalyst displayed significant increase in thermostability at 60 and 65 ☌. Fourier transform infrared spectroscopy (FTIR) analysis confirmed linkage of inulinase with chitosan particles. Inulinase was immobilized on chitosan particles and employed for fructose production by inulin hydrolysis. In order to meet the demand of concentrated fructose syrup, inulin hydrolysis at high substrate loading is essential. For commercial production, use of immobilized inulinase is advantageous as it offers reutilization of enzyme and increase in stability. Biocatalytic production of fructose from inulin employing inulinase is the most promising alternative for fructose production. Fructose, a monosaccharide, has gained wide applications in food, pharmaceutical and medical industries because of its favourable properties and health benefits.
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